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In IC and IC + RL rats, ischemia/reperfusion injury[9,10] increased and increased dramatically, respectively, whereas the MDA oxidative stress levels progressively increased, NO tissue values decreased with ligation and increased with ligation removal

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Sheared genomic DNA was purified with 0.8 × Superior AMPure XP Alternative (12601ES08, Yeasen) and end repair, dA-tail, adaptor ligation, AMPure XP bead purification, PCR enrichment, and PCR product purification were performed using the DNA Library Prep Kit (Novogene) with minor modifications to the manufacturer’s protocol