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Classically activated (M1) macrophages are usually induced by lipopolysaccharide (LPS) or interferon-γ (IFN-γ) and are characterized by high iNOS expression and increased secretion of pro-inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6)