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The mitochondrial TCA cycle plays an important role in the occurrence of cuproptosis, in which protein lipoylation is specific to the four proteins: dihydrolipoamide branched-chain transacylase E2 (DBT), glycine cleavage system protein H (GCSH), dihydrolipoamide S-succinyltransferase (DLST), and DLAT, involved in this cycle [60]

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Run these at baseline and post-protocol