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Microglial GLS1 overexpression drives neuroinflammation and induces neuronal ferroptosis To investigate the impact of microglial Gls1 overexpression on neuronal cells, we established a Transwell co-culture system, in which Gls1 -overexpressing BV2 microglia (BV2-OE) were seeded in the upper compartment and HT22 neuronal cells in the lower compartment (Fig