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Cells were lysed as previously described (Mukanganyama et al., Preparation of Alkylated GSTs Using iodo-APAP or IAM Reductive alkylation of human GSTA1, M1, M2, or P1 (1.8–3.6 nmol) was performed in 100 mM ammonium bicarbonate (ABC, pH 8.5) using DTT (250 nmol

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Mouse and cell studies have looked at clearing senescent cells in aged tissue, testosterone-producing Leydig cells, cartilage chondrocytes, and blood-vessel endothelial cells