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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

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This decrease has been associated with many common symptoms people notice over time, such as: • Persistent fatigue • Slower recovery after exercise • Brain fog • Reduced metabolic efficiency While aging is natural, supporting cellular energy production may help maintain vitality and resilience as the years go on