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At the end of the reperfusion period, all animals were euthanized by exsanguination under deep anesthesia induced with ketamine (45 mg/kg) and xylazine (5 mg/kg), administered intramuscularly into the forelimb muscles to ensure complete loss of pain and reflexes

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That rodent work is nonetheless specific, and it points at the mesolimbic reward system rather than the brainstem satiety circuits: Amylin receptors are co-expressed on dopamine neurons of the ventral tegmental area

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