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Venous whole blood samples from patients were collected into 10 ml K2EDTA Vacutainers, centrifuged at 1000g for 10 min at +4°C to collect 1,8 ml of plasma

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Size (mg):100mg

and lactatate dehydrogenase, LDH) were measured using an auto-analyzer AU-800 (Olympus, Tokyo), and troponin T was assessed by a rapid quantitative assay using the Cardiac Reader (Roche Diagnostics, Mannheim, Germany)