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In the H&E-stained sections, Various parameters, such as surface area and diameter of the seminiferous tubules (STs), as well as the height of their epithelium, were evaluated by ImageJ software (National Institute of Health, Bethesda, MD, United States) ( 2.10 Immunoblotting A tissue homogenate was prepared from the testicular tissue, and the protein content was ascertained using the Bradford Protein Assay kit (Bio Basics Inc., Ontario, Canada, Cat# SK3041)