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Evaluation of cell apoptosis by Annexin V assay To investigate the possible role of MVs treatment on apoptosis induction in fibroblasts, on days 1, 3, and 5 of culture, Annexin V-FITC and PI (EXBIO, Vestec, Czech Republic) were utilized for double-staining of cells, followed by flow cytometry (BD Biosciences, San Jose, CA, USA) analysis