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Kava enhances acetaminophen-induced cytotoxicity by increasing glutathione depletion, resulting in oxidative stress and mitochondrial dysfunction, ultimately resulting in cell death (35)

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3,3′-Dihexyloxacarbocyanineiodide [DiOC 6 (3)] was added to a final concentration of 40 nM for ΔΨm determination, 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) to 5 μM for H 2 O 2 , and MitoSOX to 1 μM for superoxide anion