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Forty microliters were added to each reaction mixture containing 0.1 M hydroxyethyl piperazine ethane sulfonicacid (HEPES) (pH 7.5) that consisted of 2 mM disodium ethylenediaminetetraacetate dihydrate (Na 2 EDTA), 1.2 mM dichlorophenolindophenol (DCPIP), 20 mM phenazine methosulfate (PMS), and 10 mM glucose-6-phosphate